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PK136

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編號:B165511

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產品名稱	PK136
商品貨號	B165511
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	Hybridoma: B lymphocyte
Product Format	frozen
Morphology	Lymphoblast-like
Culture Properties	Suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Strain	(C3H X BALB/c)F1 (B cell); BALB/c (myeloma)
Applications	The antibody is specific for NK cells and is cytotoxic in the presence of complement.
Storage Conditions	liquid nitrogen vapor phase
Derivation	Animals were immunized with spleen and bone marrow cells from CE mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against mouse natural killer (NK) cells
Cellular Products	immunoglobulin; monoclonal antibody; against mouse natural killer (NK) cells
Comments	The antibody is specific for NK cells and is cytotoxic in the presence of complement. The pattern of reactivity of the antibody with cells of various strains of mice matches the pattern of conventional NK 1.1 antisera. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing	Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10⁵viable cells/mL. Maintain cultures at a cell concentration between 1 x 10⁵ and 1 x 10⁶cells/mL. Do not allow the cell concentration to exceed 1 x 10⁶cells/mL. Medium Renewal: Every 2 to 3 days
Cryopreservation	Complete culture medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C
Isotype	IgG2a
Name of Depositor	GC Koo
Deposited As	mouse (B cell); mouse (myeloma)
References	Koo GC, et al. The NK-1.1 (-) mouse: a model to study differentiation of murine NK cells. J. Immunol. 137: 3742-3747, 1986. PubMed: 3782794 Koo GC, Peppard JR. Establishment of monoclonal anti-NK-1.1 antibody. Hybridoma 3: 301-303, 1984. PubMed: 6500587 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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