| Applications |
The antibody is specific for NP-AVP in liquid phase radioimmunoassays, but cross-reacts with all other neurophysins (murine, human, bovine, frog) when used as an immunohistochemical stain. Tested and found negative for ectromelia virus (mousepox). |
| Comments |
Animals were immunized with an acid soluble extract of rat (Sprague-Dawley) intermediate lobes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for NP-AVP in liquid phase radioimmunoassays, but cross-reacts with all other neurophysins (murine, human, bovine, frog) when used as an immunohistochemical stain. Tested and found negative for ectromelia virus (mousepox). |
| References |
Ben-Barak Y, et al. Neurophysin in the hypothalamo-neurohypophysial system. I. Production and characterization of monoclonal antibodies. J. Neurosci. 5: 81-97, 1985. PubMed: 3880813
Ben-Barak Y, et al. Phylogenetic cross-reactivities of monoclonal antibodies produced against rat neurophysin. Cell. Mol. Neurobiol. 4: 339-349, 1984. PubMed: 6085284
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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