This line produces a vector that can infect mouse cells. Psi2 DAP cells produce a vector that encodes the human placental alkaline phosphatase gene and the neomycin resistance gene. The line was derived from Psi2 cells by insertion of a recombinant retrovirus genome (DAP). Cells infected with the vector produced by Psi2 DAP cells express histochemically detectable alkaline phosphatase which can be used as a marker to follow their fate in vivo. The cells may produce helper virus. To assay for helper virus, and for other retrovirus techniques, see Cepko, C.L. Lineage analysis and immortalization of neural cells via retrovirus vectors in Neuromethods, Molecular Neurobiological Techniques 16:117-219, Boulton, A.A., Baker, G.B. and Campagnoni, A.T., Eds., Humana Press, Clifton, NJ, 1989. |
