| 產品名稱 |
PtK2 (NBL-5) |
| 商品貨號 |
B165535 |
| Organism |
Potorous tridactylus, potoroo |
| Tissue |
kidney |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Normal |
| Age |
adult |
| Gender |
male |
| Applications |
This cell line is a suitable transfection host. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Clinical Data |
male |
| Genes Expressed |
keratin |
| Cellular Products |
keratin |
| Virus Susceptibility |
Human Coxsackievirus A 9
Herpes simplex virus
Vaccinia virus
Vesicular stomatitis New Jersey virus
|
| Virus Resistance |
adenovirus 5; coxsackievirus B5; poliovirus 2 |
| Comments |
The cells are positive for keratin by immunoperoxidase staining. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin 0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37oC to facilitate dispersal.
- Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension into new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
| Name of Depositor |
KH Whalen |
| Deposited As |
Potorous tridactylis |
| References |
Walen KH. Spatial Relationships in the Replication of Chromosomal DNA. Genetics 51: 915-929, 1965. PubMed: 14337764
|
