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QT6

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編號:B165547

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產(chǎn)品名稱	QT6
商品貨號	B165547
Organism	Coturnix coturnix japonica, quail, Japanese
Cell Type	methylcholanthrene induced
Product Format	frozen
Morphology	fibroblast
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	fibrosarcoma
Applications	This cell line is a suitable transfection host.
Storage Conditions	liquid nitrogen vapor phase
Derivation	Derived from chemically induced tumors of Japanese quail.
Tumorigenic	Yes, in animal host.
Effects	Yes, in animal host and soft agar.
Virus Susceptibility	Rous sarcoma virus Avian encephalomyelitis virus Rabies virus Marek's disease virus
Complete Growth Medium	Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 10% tryptose phosphate broth and 5% bovine calf serum
Subculturing	Resuspend cells by pipetting fresh medium across the monolayer. Cells may also be dislodged by hitting culture flask against the palm of the hand. Subculture at approximately 75% confluency. Use of 0.5% polyvinylpyrrolidone (PVP) in a trypsin-EDTA solution increases the viability when subculturing by enzymatic means. Neutralize and centrifuge to remove additive. Note: When subcultures are prepared using pipetting or mechanical means the cells adhere lightly to the flask substrate and exhibit a tendency toward clumping. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week
Cryopreservation	Complete growth medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37°C
Name of Depositor	C Moscovici
Deposited As	Coturnix coturnix japonica
References	Moscovici C, et al. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail. Cell 11: 95-103, 1977. PubMed: 194709 Imagawa S, et al. Negative regulation of the erythropoietin gene expression by the GATA transcription factors. Blood 89: 1430-1439, 1997. PubMed: 9028967 Martin PT, Sanes JR. Integrins mediate adhesion to agrin and modulate agrin signaling. Development 124: 3909-3917, 1997. PubMed: 9367446 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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