| 產品名稱 |
R17 217.1.3 |
| 商品貨號 |
B165554 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension, suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Erythroleukemia |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with S194/5.XXO.BU.1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse transferrin receptor |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse transferrin receptor |
| Comments |
Animals were immunized with the mouse erythroleukemia line 745.6. Spleen cells were fused with S194/5.XXO.BU.1 myeloma cells. This antibody stains brighter by immunofluorescence than does R17 208.2 (ATCC TIB-220). The antibody does not prevent cell proliferation. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.02 mM 2-mercaptoethanol and 10% horse serum
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp4 cells/ml and do not allow the cell concentration to exceed 4 X 10 exp5 cells/ml. Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2a , IgG2a |
| Name of Depositor |
J Lesley |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Lesley J, et al. Expression of transferrin receptor on murine hematopoietic progenitors. Cell. Immunol. 83: 14-25, 1984. PubMed: 6319033
|
