| 產品名稱 |
R2-9A5 [R2-9A5.2A2.1A10] |
| 商品貨號 |
B165560 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The hybridoma cell line R2-9A5 [R2-9A5.2A2.1A10] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice. The antibody is a useful tool for detection of mouse IL-12p75 by ELISA and Western Blot analysis. |
| Derivation |
The hybridoma cell line R2-9A5 [R2-9A5.2A2.1A10] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice. |
| Clinical Data |
R2-9A5 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with recombinant mouse IL-12 p75. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p75 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p75 |
| Comments |
The hybridoma cell line R2-9A5 [R2-9A5.2A2.1A10] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice. The antibody is a useful tool for detection of mouse IL-12p75 by ELISA and Western Blot analysis. R2-9A5 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with recombinant mouse IL-12 p75. |
| Complete Growth Medium |
Iscove's Modified Dulbecco's Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol and 100 U/ml recombinant human IL-6, 90%; fetal bovine serum, 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium. Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2b |
| Name of Depositor |
DH Presky |
| Deposited As |
rat |
| Year of Origin |
1993 |
| References |
Wilkinson VL, et al. Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA. J. Immunol. Methods 189: 15-24, 1996. PubMed: 8576576
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