| 產品名稱 |
RAW 309 Cr.1 |
| 商品貨號 |
B165580 |
| Organism |
Mus musculus, mouse |
| Cell Type |
monocyte/macrophage |
| Product Format |
frozen |
| Culture Properties |
adherent |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
was derived from the same tumor as RAW 309F.1.1 (see ATCC TIB-51). The cells are negative for surface immunoglobulin (sIg-) Thy-1.2 (Thy-1.2-) and Ia (Ia-). Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
RAW 309 Cr.1 was derived from the same tumor as RAW 309F.1.1 (see ATCC TIB-51). |
| Antigen Expression |
H-2d; H-2b |
| Genes Expressed |
Abelson murine leukemia virus (infectious); lysozyme,H-2d; H-2b |
| Cellular Products |
Abelson murine leukemia virus (infectious); lysozyme |
| Comments |
RAW 309 Cr.1 was derived from the same tumor as RAW 309F.1.1 (see ATCC TIB-51). The cells are negative for surface immunoglobulin (sIg-) Thy-1.2 (Thy-1.2-) and Ia (Ia-). The cells will pinocytose neutral red and phagocytose latex beads and zymosan. They will also lyse antibody coated sheep erythrocytes. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Name of Depositor |
WC Raschke |
| Deposited As |
Mus musculus |
| References |
Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198
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