| 產品名稱 |
RTG-2 |
| 商品貨號 |
B165637 |
| Organism |
Oncorhynchus mykiss, trout, rainbow |
| Tissue |
mixed; testis; ovary |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender |
male and female mixed |
| Storage Conditions |
liquid nitrogen vapor phase |
| Virus Resistance |
poliovirus 1 |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with cold (less than 24°C) 0.25% (w/v) Trypsin - 0.03% EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.Cultures may be established with as few as 3 x 103 cells/mL.
- Incubate cultures at 22°C.
Medium Renewal: Once per week
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells: A Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley - Liss, N.Y., 2005. |
| Culture Conditions |
Temperature: 22°C; cells do not survive above 26°C but will grow at temperatures as low as 4°C. |
| Name of Depositor |
K Wolf |
| Deposited As |
Salmo gairdneri |
| References |
. . Science 135: 1065, 1962.
Officer JE. Ability of a fish cell line to support the growth of mammalian viruses. Proc. Soc. Exp. Biol. Med. 116: 190-194, 1964. PubMed: 14200103
Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741
|
| Cross References |
Nucleotide (GenBank) :
U82129
Oncorhynchus mykiss Y1 RNA, partial sequence.
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