Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
lymphoma
Strain
BALB/c
Applications
The cells are also resistant to 6-thioguanine and sensitive to thymidine, and hydrocortisone.
Tested and found negative for ectromelia virus (mousepox).
Antigen Expression
H-2d; Thy-1.2; TL
Genes Expressed
H-2d; Thy-1.2; TL
Comments
This variant of S49.1G.3 (ATCC TIB-34) was selected for resistance to 5 mcg/ml phytohemagglutinin.
The cells are also resistant to 6-thioguanine and sensitive to thymidine, and hydrocortisone.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor
P Ralph
Deposited As
Mus musculus
References
Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928
Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579
