| 產(chǎn)品名稱 |
SA22 |
| 商品貨號 |
B165660 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with rboIL-1 beta and cross-reacts with its human analog (rHuIL-1 beta). The antibodies neutralizes IL-1 activity (both macrophage derived IL-1 and recombinant IL-1 beta) in a thymocyte costimulation assay. |
| Derivation |
Animals were immunized with purified rboIL-1 beta. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibodies neutralizes IL-1 activity (both macrophage derived IL-1 and recombinant IL-1 beta) in a thymocyte costimulation assay. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against bovine recombinant interleukin 1 beta (rboIL-1 beta) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against bovine recombinant interleukin 1 beta (rboIL-1 beta) |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in pristane primed BALB/c mice |
| Comments |
Animals were immunized with purified rboIL-1 beta. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with rboIL-1 beta and cross-reacts with its human analog (rHuIL-1 beta). It does not react with other related monokines tested (ie.g., human IL-6 and TNF alpha). The antibodies neutralizes IL-1 activity (both macrophage derived IL-1 and recombinant IL-1 beta) in a thymocyte costimulation assay. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 1 mM non-essential amino acids, 0.1 mM hypoxanthine, 0.032 mM thymidine and BME vitamins, 70%; hybridoma cloning factor (Origen), 10%; fetal bovine serum, 20%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
F Blecha |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Reddy DN, et al. Isolation and characterization of monoclonal antibodies to recombinant bovine interleukin-1 beta. Vet. Immunol. Immunopathol. 36: 17-29, 1993. PubMed: 7680508
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