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Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10⁵ cells/mL and maintain between 1 X 10⁵ and 1 X 10⁶ cells/mL.

Medium Renewal: Every 2 to 3 days

Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260

Dunham LJ, Stewart HL. A survey of transplantable and transmissible animal tumors. J. Natl. Cancer Inst. 13: 1299-1377, 1953. PubMed: 13035452