| 產品名稱 |
SC-1 |
| 商品貨號 |
B165668 |
| Organism |
Homo sapiens, human |
| Cell Type |
B lymphocyte; Epstein-Barr virus (EBV) transforme |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
2 Cells Contain HERPESVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Comments |
The line is homozygous for the sickle cell allele at the hemoglobin beta chain locus. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing |
Medium Renewal: Every 3 to 4 days Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10 exp5 viable cells/ml. Maintain cell density between 5 X 10 exp5 and 1.5 X 10 exp6 viable cells/ml. |
| Cryopreservation |
Culture medium, 92.5%; DMSO, 7.5% |
| Culture Conditions |
Temperature: 37.0°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10,7.3 D13S317: 11,12 D16S539: 10,11 D5S818: 11,12 D7S820: 8,10 THO1: 7 TPOX: 6,9 vWA: 14 |
| Name of Depositor |
Cetus Corp. |
| Deposited As |
Homo sapiens |
| U.S. Patent Number |
|
| References |
Mullis KB, et al. Process for amplifying, detecting, and/or-cloning nucleic acid sequences. US Patent 4,683,195 dated Jul 28 1987
Mullis KB. Process for amplifying nucleic acid sequences. US Patent 4,683,202 dated Jul 28 1987
|
