| 產(chǎn)品名稱 |
SCaBER |
| 商品貨號 |
B165673 |
| Organism |
Homo sapiens, human |
| Tissue |
urinary bladder |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
squamous cell carcinoma |
| Age |
58 years |
| Gender |
male |
| Ethnicity |
Black |
| Karyotype |
2n = 46. The stemline chromosome number is hypotriploid with the 2S component occurring at 9.2%., Eleven markers [t(1;?) del (1)(p13), t(3;?), i(4q), t(8;?), t(9;?), M6, t(1;11), t(21;22), M13, and t(20;?)] and 1 to 3 small chromosome fragments were common to most S metaphases., At least one Y chromosome was always detectable. Number 3 was nullisomic. Neither homogenously staining regions (HSR) nor double minutes (DM) were seen. |
| Clinical Data |
58 years Black male |
| Tumorigenic |
Yes |
| Effects |
Yes, epidermoid carcinoma forms in nude mice |
| Comments |
Contains the unusual type A isoenzyme of glucose-6-phosphate dehydrogenase. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% This medium is formulated for use with a 5% CO2 in air atmosphere. ATCC tested fetal bovine serum is available as ATCC? Catalog No. 30-2020 (500 mL) and ATCC? Catalog No. 30-2021 (100 mL).
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| Subculturing |
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 11,13 D13S317: 11,12 D16S539: 11,12 D5S818: 12,13 D7S820: 8,10 THO1: 7,9 TPOX: 9,11 vWA: 16 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, A GLO-I, 1-2 Me-2, 2 PGM1, 1-2 PGM3, 1-2 |
| Name of Depositor |
C O'Toole |
| References |
O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.
O'Toole C, et al. A cell line (SCABER) derived from squamous cell carcinoma of the human urinary bladder. Int. J. Cancer 17: 707-714, 1976. PubMed: 947851
Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
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