| 產品名稱 | SCC-25 |
|---|---|
| 商品貨號 | B165674 |
| Organism | Homo sapiens, human |
| Tissue | tongue |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | squamous cell carcinoma |
| Age | 70 years |
| Gender | male |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Clinical Data | male
70 years |
| Genes Expressed | epidermal keratins; low levels of involucrin |
| Cellular Products | epidermal keratins; low levels of involucrin |
| Tumorigenic | Yes |
| Effects | Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Comments | SCC-25 cells are capable of only limited proliferation in semi-solid medium. |
| Complete Growth Medium | A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate and supplemented with 400 ng/ml hydrocortisone and 10% fetal bovine serum
|
| Subculturing | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Note: Cells are disaggregated to single cells when the culture is preconfluent. They do not require a feeder layer. |
| Cryopreservation | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile | Amelogenin: X CSF1PO: 10 D13S317: 13 D16S539: 11,12 D5S818: 12 D7S820: 12 THO1: 8 TPOX: 8,12 vWA: 17,19 |
| Name of Depositor | JG Rheinwald |
| Deposited As | Homo sapiens |
| References | Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
