Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
rhabdomyosarcoma
Age
17 years
Gender
male
Ethnicity
Caucasian
Derivation
The line was established from cells from the bone marrow of a child with rhabdomyosarcoma.
Derived from metastatic site, bone marrow
Clinical Data
17 years
Caucasian
male
Oncogene
gli + (amplified 30 fold), N-myc + (not amplified)
Genes Expressed
gli + (amplified 30 fold), N-myc + (not amplified)
Comments
The cells show ultrastructural elements of primitive skeletal muscle differentiation.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Twice per week
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
D5S818: 12, 13
D13S317: 11
D7S820: 10
D16S539: 12
THO1: 9, 9.3
TPOX: 8, 11
vWA: 17, 18
CSF1PO: 10, 11
Amelogenin: XY
Name of Depositor
DN Shapiro
Deposited As
Homo sapiens
References
Oliner JD, et al. Amplification of a gene encoding a p53-associated protein in human sarcomas. Nature 358: 80-83, 1992. PubMed: 1614537
Roberts WM, et al. Amplification of the gli gene in childhood sarcomas. Cancer Res. 49: 5407-5413, 1989. PubMed: 2766305
Douglass EC, et al. A specific chromosomal abnormality in rhabdomyosarcoma [published erratum appears in Cytogenet Cell Genet 1988;47(4):following 232]. Cytogenet. Cell Genet. 45: 148-155, 1987. PubMed: 3691179
