| 產品名稱 |
SK-CO-1 |
| 商品貨號 |
B165700 |
| Organism |
Homo sapiens, human |
| Tissue |
colon; derived from metastatic site: ascites |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
colorectal adenocarcinoma |
| Age |
65 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
(P7) hypertriploid to hypotetraploid with abnormalities including dicentrics, minutes, rings, secondary constrictions, and 8 large submetacentric markers |
| Clinical Data |
65 years Caucasian male |
| Antigen Expression |
Blood Type O; Rh+; HLA A1, A3, B7, B13 |
| Oncogene |
myc +; ras +; myb +; fos +; sis +; p53 +; abl -; ros -; src - |
| Genes Expressed |
myc +; ras +; myb +; fos +; sis +; p53 +; abl -; ros -; src - |
| Comments |
Ultrastructural features include microvilli, microfilaments, small mitochondria with dark granules and dilated cristae, sparse RER, few Golgi, lipid droplets, multilamellar bodies, no virus particles were detected. The line is positive for expression of c-myc, N-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 13 D13S317: 14 D16S539: 13 D5S818: 9,11 D7S820: 8,10 THO1: 8,9 TPOX: 8,11 vWA: 18,19 |
| Isoenzymes |
AK-1, 1-2 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1-2 |
| Name of Depositor |
G Trempe, LJ Old |
| Deposited As |
Homo sapiens |
| References |
Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212
Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066
Kutchera W, et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486
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