| 產品名稱 | SKO-007 |
|---|---|
| 商品貨號 | B165720 |
| Organism | Homo sapiens, human |
| Cell Type | B lymphocyte |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation | This line was derived from the U266 myeloma cell line of Nilsson Ref   Olsson L, Kaplan HS. Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity. Proc. Natl. Acad. Sci. USA 77: 5429-5431, 1980. PubMed: 6159646 by selecting for resistance to 8-azaguanine; the cells cells are HAT sensitive. |
| Antigen Expression | HLA A2, A3, B7, Bw60, Bw6, Cw3 |
| Genes Expressed | immunoglobulin; monoclonal antibody |
| Cellular Products | immunoglobulin; monoclonal antibody |
| Comments | This is a batch of SKO-007 (formerly available as ATCC CRL-8033) that was cured at the ATCC of a mycoplasma contamination.
The J3 clone of SKO-007 is available also (ATCC CRL-8033-2). |
| Complete Growth Medium | RPMI 1640 medium with 0.02 mg/ml 6-thioguanine, 1 mM sodium pyruvate and 2 mM L-glutamine, 85%; fetal bovine serum, 15%
|
| Subculturing | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: 2 to 3 times per week |
| Cryopreservation | Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 12,13 D13S317: 12 D16S539: 10 D5S818: 11,12 D7S820: 11,12 THO1: 5,7 TPOX: 8 vWA: 17 |
| Isotype | IgE; lambda light chain |
| Name of Depositor | H Kaplan, Stanford University Board of Trustees |
| Deposited As | Homo sapiens |
| U.S. Patent Number | |
| References | Olsson L, Kaplan HS. Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity. Proc. Natl. Acad. Sci. USA 77: 5429-5431, 1980. PubMed: 6159646 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
