| 產(chǎn)品名稱 | SL-29 |
|---|---|
| 商品貨號 | B165729 |
| Organism | Gallus gallus, chicken |
| Tissue | embryo |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | embryo; 11 days gestation |
| Applications | This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | This cell line was developed by standard trypsinization of a decapitated 11-day SPAFAS leghorn embryo. |
| Comments | The cells have a doubling potential of 35 population doublings. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: tryptose phosphate broth to a final concentration of 5% and fetal bovine serum to a final concentration of 5%.
|
| Subculturing | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: An inoculation density of 5 X 104 viable cells per cm2 of flask or dish surface area is recommended
Medium Renewal: 2 to 3 times per week
Note: The tryptose phosphate broth should be prepared fresh every 2 weeks. Fresh glutamine is also very important for the growth of this line. |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Population Doubling Capacity | The cells have a doubling potential of 35 population doublings. |
| Name of Depositor | RJ Hay |
| Deposited As | Gallus gallus |
| References | . Aging in cell and tissue cultures. New York: Plenum Press; 1970. Ramirez AD, et al. Antisporozoite antibodies with protective and nonprotective activities: in vitro and in vivo correlations using Plasmodium gallinaceum, an avian model. J. Eukaryot. Microbiol. 42: 705-708, 1995. PubMed: 8520586 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
