| 產品名稱 |
Sp2/01-Ag |
| 商品貨號 |
B165747 |
| Organism |
Mus musculus, mouse |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 3 to 4 days
Note: It is recommended that the cells be cultured in the presence of mouse peritoneal macrophages. |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor |
Hoffmann-La Roche Ltd. |
| Deposited As |
Mus musculus |
| U.S. Patent Number |
|
| References |
Rotter V, et al. Abelson murine leukemia virus-induced tumors elicit antibodies against a host cell protein, P50. J. Virol. 36: 547-555, 1980. PubMed: 6159484
|
