Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
alveolar cell carcinoma
Age
44 years
Gender
female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor temperature
Derivation
The SW 1573 line was established in 1977 by A. Leibovitz from an alveolar cell carcinoma.
Clinical Data
female
44 years
Caucasian
Antigen Expression
Blood Type O; Rh +
Comments
The SW 1573 line was established in 1977 by A. Leibovitz from an alveolar cell carcinoma.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C
Subculture Ratio: 1:4 to 1:10
Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Atmosphere: air, 100%
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 12,14
D5S818: 12,13
D7S820: 9,11
THO1: 6,9.3
TPOX: 8,11
vWA: 20
Name of Depositor
W McCombs
Deposited As
Homo sapiens
References
Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474
