| 產品名稱 |
SW 982 [SW-982, SW982] |
| 商品貨號 |
B165790 |
| Organism |
Homo sapiens, human |
| Tissue |
synovium |
| Product Format |
frozen |
| Morphology |
mixed |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
synovial sarcoma |
| Age |
25 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
Hyperdiploid; modal number = 48; range = 42 to 58. The rate of higher ploidies was 1.6%. Nine markers were common to all cells. These were: t(1q4p), del(5) (q31;q33), der(9) t(4;9) (q11;p24), t(8q12p), t(9q13q) and four others.Double minutes (DM) were seen in some cells (usually only one copy). Normal N9 was absent, N4, N8, and N13 were consistently single-copied and the X was double-copied. |
| Derivation |
The SW 982 cell line was initiated by A. Leibovitz in 1974 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a biphasic synovial sarcoma removed from a 25 year old female Caucasian. |
| Clinical Data |
25 years Caucasian female The SW 982 cell line was initiated by A. Leibovitz in 1974 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a biphasic synovial sarcoma removed from a 25 year old female Caucasian. |
| Comments |
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C
Atmosphere: air, 100% |
| STR Profile |
Amelogenin: X CSF1PO: 11,12 D13S317: 12,13 D16S539: 11,12 D5S818: 11,13 D7S820: 9,11 THO1: 9.3 TPOX: 9,11 vWA: 19,20 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 PGM1, 1-2 PGM3, 1-2 |
| Name of Depositor |
A Leibovitz |
| Deposited As |
Homo sapiens |
| Passage History |
An ampule at passage 4 was received at the ATCC in January, 1982. |
| References |
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
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