| 產品名稱 |
SW1463 [SW 1463, SW-1463] |
| 商品貨號 |
B165794 |
| Organism |
Homo sapiens, human |
| Tissue |
rectum |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Dukes' type C, colorectal adenocarcinoma |
| Age |
66 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The line was derived from a grade II mucous producing and grade III solid tumor. |
| Clinical Data |
66 years Caucasian female |
| Antigen Expression |
blood type A; Rh + |
| Genes Expressed |
carcinoembryonic antigen (CEA) 223 ng/106 cells/10 days; keratin |
| Cellular Products |
carcinoembryonic antigen (CEA) 223 ng/106 cells/10 days; keratin |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments |
CSAp negative (CSAp-).
The cells are positive for keratin by immunoperoxidase staining.
Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture just prior to confluency
-
Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mof complete growth medium and aspirate cells by gently pipetting.
- To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube and spin at approximately 125 x g for 5 to10 minutes.
- Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
-
Incubate cultures at 37°C without CO2.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 1 to 2 times per week
|
| Culture Conditions |
Atmosphere: Air, 100% |
| STR Profile |
Amelogenin: X CSF1PO: 11,12 D13S317: 12,13 D16S539: 11 D5S818: 13,14 D7S820: 9 THO1: 6,7 TPOX: 8,11 vWA: 16 |
| Isoenzymes |
ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 1-2 |
| Name of Depositor |
A Leibovitz |
| Deposited As |
Homo sapiens |
| References |
Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501
|
