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SWLA3

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編號:B165806

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產品名稱	SWLA3
商品貨號	B165806
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension (single cells and clusters); some cells may attach to the culture vessel
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Strain	Strain: BALB/c (B cell); BALB/c (myeloma)
Applications	The SWLA1 (ATCC HB-12559), SWLA2 (ATCC HB-12580), and SWLA3 (ATCC HB-12558) antibodies appear to recognize a species-specific lipooligosaccharide or lipopolysaccharide on the cell surface of S. mutans. These antibodies can be used in the clinical diagnosis and treatment of dental caries in humans.
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation	Spleen cells were fused with NS-1-Ag4-1 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against Streptococcus mutans
Cellular Products	immunoglobulin; monoclonal antibody; against Streptococcus mutans
Comments	Animals were immunized with Streptococcus mutans Clarke (ATCC 25175). Spleen cells were fused with NS-1-Ag4-1 myeloma cells. The SWLA1 (ATCC HB-12559), SWLA2 (ATCC HB-12580), and SWLA3 (ATCC HB-12558) antibodies appear to recognize a species-specific lipooligosaccharide or lipopolysaccharide on the cell surface of S. mutans. These antibodies have substantially no reactivity with any of the following bacterial strains: Streptococcus rattus (ATCC 19645, Streptococcus gordonii (ATCC 10558), Streptococcus gordonii (ATCC 13396), Streptococcus mitis (ATCC 49456). Also Streptococcus sobrinus (ATCC 33478), Streptococcus sobrinus 6715, Streptococcus sanguis (ATCC 10556), Streptococcus sanguis (ATCC 49295), Streptococcus anginosus (ATCC 33397), Lactobacillus acidophilus (ATCC 4356), Lactobacillus casei (ATCC 4646), Actinobacillus actinomycetemcomitans (ATCC 33384), Porphyromonas gingivalis (ATCC 33277), Prevotella intermedia (ATCC 49046), Bacteroides forsythus (ATCC 43047), Eikenella corrodens (ATCC 23834), Fusobacterium nucleatum (ATCC 25586), Treponema denticola (ATCC 33520), Campylobacter rectus (ATCC 33238), Myxococcus xanthus DZ2, and Escherichia coli HB101. These antibodies can be used in the clinical diagnosis and treatment of dental caries in humans.
Complete Growth Medium	HL-1 medium supplemented with 4 mM L-glutamine, 1 mM sodium pyruvate, 10 mM HEPES and 10% fetal bovine serum. HL-1 medium can be obtained from Lonza (catalog number 77201).
Subculturing	Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Note: About 50% of the cells may attach. Cells in suspension grow singly and in small clusters.
Cryopreservation	culture medium, 45%; fetal bovine serum, 45%; DMSO, 10%
Culture Conditions	Temperature: 37.0°C
Name of Depositor	Regents of the University of California
Deposited As	mouse (B cell); mouse (myeloma)
U.S. Patent Number	6,231,857
References	Shi W, Hume WR. Antibodies to S. mutans and uses thereof. US Patent 6,231,857 dated May 15 2001

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