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T11D7e2

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編號(hào):B165811

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產(chǎn)品名稱	T11D7e2
商品貨號(hào)	B165811
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Storage Conditions	liquid nitrogen vapor phase
Derivation	Animals were immunized with W/Fu rat thymocytes. Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against Thy-1.1 antigen; cytotoxic
Cellular Products	immunoglobulin; monoclonal antibody; against Thy-1.1 antigen; cytotoxic
Comments	Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 10⁵ viable cells/mL. Maintain cultures at a cell concentration between 1 x 10⁵ and 1 x 10⁶ cells/mL. Do not allow the cell concentration to exceed 1 x 10⁶ cells/mL. Medium Renewal: Every 2 to 3 days
Cryopreservation	50% FBS, 40% complete growth medium, 10% DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: 5% CO₂
Isotype	IgM
Name of Depositor	PA Lake
Deposited As	mouse (B cell); mouse (myeloma)
References	Lake P, et al. Production and characterization of cytotoxic Thy-1 antibody-secreting hybrid cell lines. Detection of T cell subsets. Eur. J. Immunol. 9: 875-886, 1979. PubMed: 393521 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

序號(hào)	文章標(biāo)題	操作
1	ATCC細(xì)胞運(yùn)輸和處理	查看詳情
2	其他海外品牌代理	查看詳情
3	ATCC代理	查看詳情
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梅經(jīng)理	17280875617	1438578920
胡經(jīng)理	13345964880	2438244627
周經(jīng)理	17757487661	1296385441
于經(jīng)理	18067160830	2088210172
沈經(jīng)理	19548299266	2662369050
李經(jīng)理	13626845108	972239479