| 產(chǎn)品名稱(chēng) |
T3-3A1 [3A1] |
| 商品貨號(hào) |
B165815 |
| Organism |
Mus musculus, mouse |
| Tissue |
spleen |
| Cell Type |
hybridoma: B lymphocyte; somatic cell hybri |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
identifying T cell acute lymphoblastic leukemia.,identifying T cell acute lymphoblastic leukemia. |
| Age |
adult |
| Strain |
|
| Applications |
The antibody recognizes a 40000 dalton cell surface antigen that is an early marker of the T cell lineage and may be useful for identifying T cell acute lymphoblastic leukemia. This line is derived from the same fusion used to produce ATCC HB-21 and ATCC HB-22. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
This line is derived from the same fusion used to produce ATCC HB-21 and ATCC HB-22. |
| Comments |
The antibody recognizes a 40000 dalton cell surface antigen that is an early marker of the T cell lineage and may be useful for identifying T cell acute lymphoblastic leukemia. This line is derived from the same fusion used to produce ATCC HB-21 and ATCC HB-22. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) cells/ml Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
mouse IgG1 kappa |
| Name of Depositor |
A Fauci, BF Haynes |
| Deposited As |
Mus musculus |
| References |
Haynes BF, et al. Human lymphocyte antigens: production of a monoclonal antibody that defines functional thymus-derived lymphocyte subsets. Proc. Natl. Acad. Sci. USA 76: 5829-5833, 1979. PubMed: 316538
Eisenbarth GS, et al. Production of monoclonal antibodies reacting with peripheral blood mononuclear cell surface differentiation antigens. J. Immunol. 124: 1237-1244, 1980. PubMed: 6153676
Haynes BF, et al. Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans. Proc. Natl. Acad. Sci. USA 77: 2914-2918, 1980. PubMed: 6156457
Prieyl JA, LeBien TW. Interleukin 7 independent development of human B cells. Proc. Natl. Acad. Sci. USA 93: 10348-10353, 1996. PubMed: 8816803
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