| 產品名稱 |
TE7 |
| 商品貨號 |
B165847 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with cells in the thymic capsule and interlobular septae and vessels. It also reacts with non-endocrine fibrous stroma in the thymus, and with mesodermally derived connective tissue in other parts of the body. It defines the mesodermal component of the human thymic microenvironment that most likely induces thymic epithelial differentiation early in the development of the thymus. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. It also reacts with non-endocrine fibrous stroma in the thymus, and with mesodermally derived connective tissue in other parts of the body. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human thymus |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human thymus |
| Comments |
Animals were immunized with human thymic stroma. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with cells in the thymic capsule and interlobular septae and vessels. It also reacts with non-endocrine fibrous stroma in the thymus, and with mesodermally derived connective tissue in other parts of the body. It defines the mesodermal component of the human thymic microenvironment that most likely induces thymic epithelial differentiation early in the development of the thymus. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%;
fetal bovine serum, 20%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
BF Haynes |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Haynes BF, et al. Phenotypic characterization and ontogeny of mesodermal-derived and endocrine epithelial components of the human thymic microenvironment. J. Exp. Med. 159: 1149-1168, 1984. PubMed: 6200562
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