| 產品名稱 |
THB-5 [HB-5, HB5] |
| 商品貨號 |
B165859 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with the human C3d receptor (14500 dalton). Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human C3d receptor (CR2, Epstein-Barr virus (EBV) receptor); against CD21 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human C3d receptor (CR2, Epstein-Barr virus (EBV) receptor); against CD21 |
| Comments |
Animals were immunized with the SB human B cell line. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with the human C3d receptor (14500 dalton). This receptor also is the receptor for Epstein-Barr virus (EBV) on human B cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: RPMI medium 1640, 85%; fetal bovine serum, 15%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a; kappa light chain |
| Name of Depositor |
TF Tedder |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Weis JJ, et al. Identification of a 145,000 Mr membrane protein as the C3d receptor (CR2) of human B lymphocytes. Proc. Natl. Acad. Sci. USA 81: 881-885, 1984. PubMed: 6230668
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