| 產品名稱 |
THB-7 [HB-7, HB7] |
| 商品貨號 |
B165860 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with a 45000 dalton OKT-10 like molecule on immature human lymphocytes, plasma cells and activated T cells. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against a human OKT-10 like molecule; against CD38 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against a human OKT-10 like molecule; against CD38 |
| Comments |
Animals were immunized with the BJAB human B cell line. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with a 45000 dalton OKT-10 like molecule on immature human lymphocytes, plasma cells and activated T cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
RPMI 1640 medium, 85%; fetal bovine serum, 15%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Isotype |
IgG1 |
| Name of Depositor |
TF Tedder |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Tedder TF, et al. Discontinuous expression of a membrane antigen (HB-7) during B lymphocyte differentiation. Tissue Antigens 24: 140-149, 1984. PubMed: 6440308
|
