| 產品名稱 |
TM1 |
| 商品貨號 |
B165869 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain:? BALB/c (B cell); BALB/c (myeloma) |
| Applications |
Animals were immunized with human leukocytes (HLA phenotype A2,A28,Bw4,B27,B44,Cw2). Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Leu-5; cytotoxic |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Leu-5; cytotoxic |
| Comments |
Animals were immunized with human leukocytes (HLA phenotype A2,A28,Bw4,B27,B44,Cw2). Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells. The antibody is cytotoxic for Leu-5 positive (Leu-5+) cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Modified RPMI 1640 medium (see fact sheet), 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgM |
| Name of Depositor |
F Grumet |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Grumet FC, et al. A simplified new method for HLA-DR typing using the TM1 monoclonal antibody. Hum. Immunol. 6: 63-73, 1983. PubMed: 6601099
|
