| 產品名稱 |
UACC-812 |
| 商品貨號 |
B165901 |
| Organism |
Homo sapiens, human |
| Tissue |
mammary gland; breast |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
ductal carcinoma |
| Age |
43 years |
| Gender |
female |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
range = 58 to 64; abnormal banding region in 3p |
| Derivation |
This cell line was established by A. Liebovitz and associates in 1986 from breast tissue removed at mastectomy to remove a ductal carcinoma (stage II, grade IV). |
| Clinical Data |
female Prior to surgery, the patient had received extensive chemotherapy. |
| Receptor Expression |
estrogen receptor (negative), progesterone receptor (negative) |
| Comments |
Prior to surgery, the patient had received extensive chemotherapy. The cells exhibit a 15 fold amplification of the HER-2/neu oncogene sequence. They are negative for estrogen receptors, progesterone receptors and P glycoprotein. |
| Complete Growth Medium |
Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with 20 ng/ml human EGF and 20% fetal bovine serum. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Note: The cells grow very slowly, and growth is enhanced by using 20% fetal bovine serum and adding epidermal growth factor (20 ng/mL) to the medium. |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 100%
Temperature: 37°C |
| STR Profile |
Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 8, 11
D5S818: 11, 12
D7S820: 9
THO1: 9
TPOX: 8, 11
vWA: 16 |
| Population Doubling Time |
100 hrs |
| Name of Depositor |
A Liebovitz, J Trent |
| Deposited As |
Homo sapiens |
| Year of Origin |
1986 |
| References |
Proc. Am. Assoc. Cancer Res. 29: 24, 1988.
Meltzer P, et al. Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification. Br. J. Cancer 63: 727-735, 1991. PubMed: 1674877
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