| 產品名稱 |
VM-2 |
| 商品貨號 |
B165928 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody binds to a cell surface antigen present on and characteristic of human basal cells and malignant squamous cells. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human basal cells (skin) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human basal cells (skin) |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in BALB/c mice |
| Comments |
Animals were immunized with human epidermal cells. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. The antibody binds to a cell surface antigen present on and characteristic of human basal cells and malignant squamous cells. The antibody does not react with normal human fibroblasts, endothelial or hematopoietic cells. |
| Complete Growth Medium |
Culture medium: Dulbecco's modified Eagle's medium with 4.5 g/L
glucose, 0.45 mM sodium pyruvate and 0.01 M HEPES, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium, 90%; DMSO, 10% |
| Isotype |
IgG1 |
| Name of Depositor |
Stanford University |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Morhenn V. Monoclonal antibody specific for human basal cell surface antigen. US Patent 4,886,745 dated Dec 12 1989
|
