| 產品名稱 |
WAPS 12.2 |
| 商品貨號 |
B165934 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human P-selectin (CD62P) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human P-selectin (CD62P) |
| Comments |
Animals were immunized with P-selectin transfected mouse L1-2 pre-B cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody blocks leukocyte adhesion to P-selectin and to platelets. It recognizes P-selectin of a variety of mammals. |
| Complete Growth Medium |
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain
1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium
pyruvate supplemented with 0.01 mM non-essential amino acids and 0.05 mM
2-mercaptoethanol, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG1 |
| Name of Depositor |
EC Butcher |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Jutila MA, et al. Cell surface P- and E-selectin support shear-dependent rolling of bovine gamma/delta T cells. J. Immunol. 153: 3917-3928, 1994. PubMed: 7523509
|
