| 產(chǎn)品名稱 | WEHI-231 |
|---|---|
| 商品貨號 | B165940 |
| Organism | Mus musculus, mouse |
| Cell Type | B lymphocyte, immature |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension, multicell aggregates |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | B cell lymphoma |
| Strain | (BALB/c x NZB)F1 |
| Applications | This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | Mineral oil induced tumor in (BALB/c x NZB)F1 mice |
| Genes Expressed | immunoglobulin |
| Cellular Products | immunoglobulin |
| Comments | WEHI-231 cells do not secrete IgM into medium unless stimulated with lipopolysaccharide (0.01 to 3 μg/mL). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Optimum culture recovery from a frozen vial is in a T25 flask at a density between 2 to 5 X 105 viable cells/mL. If recovered at 5 X 105 viable cells/mL, media will need to be added to the culture within the first 3 days, before the density reaches 1 X 106 viable cells/mL, at which point the viability drops drastically.
After cells recover, expansions can be done at 1 X 105 viable cells/mL by adding media to decrease the cell concentration. This cell line forms tight clusters of viable cells accompanied by some single non-viable cells plus debris in suspension. With increased growth the clusters become larger. As the cell density nears 1 X 106 cells/mL, the single (non-clustered) and non-viable cells increase along with the amount of debris. At subculture, break up clusters by gentle pipetting. Established cultures can be grown in T75 flasks. Subcultivation Ratio: Add fresh medium every 2 to 3 days (depending on cell density)
Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Isotype | IgM , IgM (surface) |
| Population Doubling Time | about 20 hours |
| Name of Depositor | NL Warner, LL Lanier |
| Deposited As | Mus musculus |
| References | Boyd AW, et al. The regulation of growth and differentiation of a murine B cell lymphoma. I. Lipopolysaccharide induced differentiation. J. Immunol. 126: 2461-2465, 1981. PubMed: 6785355 Lanier LL, Warner NL. Cell cycle related heterogeneity of Ia antigen expression on a murine B lymphoma cell line:analysis by flow cytometry. J. Immunol. 126: 626-631, 1981. PubMed: 6969756 Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803 mineral oil induced tumor in (BALB/c x NZB)F1 mice To secrete IgM into the medium, these cells need to be stimulated with lipopolysaccharide (0.01 to 3 mcg/ml). |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
