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WEHI-3

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編號:B165944

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產品名稱	WEHI-3
商品貨號	B165944
Organism	Mus musculus, mouse
Tissue	peripheral blood
Product Format	frozen
Culture Properties	suspension (some adherent cells)
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	leukemia
Strain	BALB/c
Applications	Tested and found negative for ectromelia virus (mousepox). The cells exhibit only weak effector activity in antibody dependent cell mediated cytotoxicity.
Storage Conditions	liquid nitrogen vapor phase
Receptor Expression	complement (C3)
Genes Expressed	lysozyme; granulocyte colony stimulating activity (CSA); interleukin-3 (interleukin 3, IL-3)
Cellular Products	lysozyme; granulocyte colony stimulating activity (CSA); interleukin-3 (interleukin 3, IL-3)
Comments	The growth of WEHI-3 is inhibited by 4 ng/mL LPS and blocked by higher concentrations. Dextran sulfate at 30 to 40 µg/mL also inhibits growth. Latex beads are phagocytized but are not toxic. Zymosan and BCG are phagocytized and block growth. The cells exhibit only weak effector activity in antibody dependent cell mediated cytotoxicity. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: 0.05 mM 2-mercaptoethanol fetal bovine serum to a final concentration of 10%
Subculturing	Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10⁵ viable cells/mL. Maintain cell density between 2 X 10⁵ and 2 X 10⁶ viable cells/mL. Adherent cells may be harvested by scraping. Medium Renewal: Every 2 to 3 days
Cryopreservation	culture medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37°C
Name of Depositor	M Cohn
Deposited As	Mus musculus
References	Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890 Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031 Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922 McKean DJ, et al. Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells. J. Exp. Med. 154: 1419-1431, 1981. PubMed: 6170720 Boring L, et al. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors. J. Biol. Chem. 271: 7551-7558, 1996. PubMed: 8631787

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