| 產品名稱 |
WISH |
| 商品貨號 |
B165953 |
| Organism |
Homo sapiens, human |
| Tissue |
HeLa contaminant |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. |
| Antigen Expression |
The cells are positive for keratin by immunoperoxidase staining. |
| HeLa Markers |
Y |
| Genes Expressed |
The cells are positive for keratin by immunoperoxidase staining.
The cells and tumors formed from the cells are positive for Dopa decarboxylase and are negative for the TAG-72 antigen. |
| Cellular Products |
keratin |
| Virus Susceptibility |
Human poliovirus 1
Human poliovirus 2
Human adenovirus 3
Vesicular stomatitis virus
|
| Comments |
NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination
|
| Complete Growth Medium |
EMEM (30-2003) supplemented with 10% FBS (30-2020) |
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
-
Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
-
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Twice per week
|
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 9,10 D13S317: 13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
| Isoenzymes |
G6PD, A |
| Name of Depositor |
L Hayflick |
| Deposited As |
Homo sapiens |
| References |
Hayflick L. The establishment of a line (WISH) of human amnion cells in continuous cultivation. Exp. Cell Res. 23: 14-20, 1961. PubMed: 13712490
Buynak EB, et al. Infectivity Stability of Live Measles-Virus Vaccine. Am. J. Dis. Child. 103:460, 1962.
Hulkower KI, et al. Induction of prostaglandin H synthase-2 and tumor necrosis factor-alpha in human amnionic WISH cells by various stimuli occurs through distinct intracellular mechanisms. J. Pharmacol. Exp. Ther. 280: 1065-1074, 1997. PubMed: 9023325
Lundgren DW, et al. Hypotonic stress increases cyclooxygenase-2 expression and prostaglandin release from amnion-derived WISH cells. J. Biol. Chem. 272: 20118-20124, 1997. PubMed: 9242685
Perkins DJ, Kniss DA. Rapid and transient induction of cyclo-oxygenase 2 by epidermal growth factor in human amnion-derived WISH cells. Biochem. J. 321: 677-681, 1997. PubMed: 9032453
|
