| 產(chǎn)品名稱 |
WSS-1 [WS-1] |
| 商品貨號 |
B165961 |
| Organism |
Homo sapiens, human |
| Tissue |
kidney |
| Cell Type |
Transformed with adenovirus 5 DNA |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
2 [Cells contain Adenovirus]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
fetus |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
This line was derived from the human embryonic kidney line, 293 (see ATCC CRL-1573). 293 cells were transfected with an expression plasmid containing cDNA encoding the rat GABA-A receptor alpha 1, beta 2 and gamma 2 subunits. Genomic integration of the transfected alpha and gamma subunit genes was confirmed; however, the beta subunit sequences were not detected in Northern and Southern blots.
Stable transfectant expressing a type I GABAA receptor (alpha1 and gamma2 subunits). |
| Receptor Expression |
vitronectin; gamma-aminobutyric acid - A receptor (GABA-A, Type 1 BzR) |
| Genes Expressed |
gamma-aminobutyric acid (GABA) A receptor, alpha 1 [GABRA1],gamma-aminobutyric acid (GABA) A receptor, alpha 1 |
| Comments |
Produces protein gamma-aminobutyric acid (GABA) A receptor, alpha 1
Handle as potentially biohazardous material under at least Biosafety Level 2 containment.
Stable transfectant expressing a type I GABAA receptor (alpha1 and gamma2 subunits). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM L-glutamine modified to contain 4.5 g/L glucose, 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
|
| Subculturing |
Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week |
| Cryopreservation |
Freeze Medium: Culture medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 12 D13S317: 12,14 D16S539: 9,13 D5S818: 8 D7S820: 11,12 THO1: 7,9.3 TPOX: 11 vWA: 16,19 |
| Name of Depositor |
G Wong |
| Deposited As |
Homo sapiens |
| References |
Wong G, et al. Stable expression of type I gamma-aminobutyric acidA/benzodiazepine receptors in a transfected cell line. Mol. Pharmacol. 42: 996-1003, 1992. PubMed: 1336119
|
