Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The cells are resistant to 8-azaguanine, are HAT sensitive and can be used as a fusion partner for rat B cells to make rat - rat hybridomas.
Derivation
This line was produced by fusing Y3/Ag1.2.3 (ATCC CRL-1631) cells with spleen cells from an AO strain rat.
Comments
Tested and found negative for ectromelia virus (mousepox).
The cells do not secrete either heavy or light immunoglobulin chains.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/mL and maintain at or below 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete growth medium 95%; DMSO 5% (v/v). Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.
Name of Depositor
C Milstein
Deposited As
Rattus sp.
References
Kilmartin JV, et al. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J. Cell Biol. 93: 576-582, 1982. PubMed: 6811596
