| 產(chǎn)品名稱 | ZEM2S |
|---|---|
| 商品貨號 | B165991 |
| Organism | Danio rerio, zebrafish |
| Tissue | embryo |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | embryo; blastula |
| Applications | These cells provide an in vitro assay system for the comparison of enhancer/promotor activities in early stage zebrafish embryos.
The cells may provide an in vitro assay system for the study of extracelluar factors which induce and regulate cell differentiation.
|
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | ZEM2S was derived from the ZEM2 cell line that had been established from zebrafish embryos using a complex growth medium supplemented with insulin, trout embryo extract, trout and fetal bovine sera.
ZEM2S cells were derived from ZEM2 in 1993 by selection for growth in a basal nutrient medium supplemented with 5 to 10% heat-inactivated fetal bovine serum. |
| Comments | Medium conditioned by cells from the buffalo rat liver cell line available as BRL 3A (see ATCC CRL-1442). Both transient and stable expression of foreign genes have been demonstrated in transfected zebrafish blastula derived cell cultures. A culture submitted to the ATCC in October 1994 was found to be contaminated with mycoplasma, and progeny were cured by a 21 day treatment with BM Cycline. |
| Complete Growth Medium | 50% Leibovitz's L-15 medium (ATCC 30-2008) 35% Dulbecco's Modified Eagle's medium, high glucose (GIBCO 12100) 15% F12 medium (GIBCO 21700) The above are all without sodium bicarbonate Supplemented with: 0.18 g/L sodium bicarbonate 15 mM HEPES 10% heat-inactivated fetal bovine serum |
| Subculturing | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week |
| Cryopreservation | Freeze medium: Complete growth medium, 85%; additional HI-FBS, 10%, DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 28°C
Max Temperature: 29°C
Min Temperature: 26°C |
| Name of Depositor | DW Barnes |
| Deposited As | Brachydanio rerio |
| Year of Origin | 1993 |
| References | Collodi P, et al. Culture of cells from zebrafish (Brachydanio rerio) embryo and adult tissues. Cell Biol. Toxicol. 8: 43-61, 1992. PubMed: 1591622 Ghosh C, Collodi P. Culture of cells from zebrafish (Brachydanio rerio) blastula-stage embryos. Cytotechnology 14: 21-26, 1994. PubMed: 7765109 Bradford CS, et al. Cell Cultures from zebrafish embryos and adult tissues. J. Tissue Culture Methods 16: 99-107, 1994. |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
