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<em>Curvularia pseudobrachyspora </em>Marín et al.

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產(chǎn)品名稱	Curvularia pseudobrachyspora Marín et al.
商品貨號(hào)	B166093
Deposited As	Curvularia lunata (Wakker) Boedijn
Strain Designations	NRRL 2380 [CBS 215.54, IFO 6299, IMI 61535, QM 120h]
Application	Degrades insect moulting hormones Hydroxylates 11-desoxy-17-hydroxycorticosterone cortexolone, substance S Hydroxylates cytochrome P450, subfamily XIB, polypeptide 2 (steroid-11-beta-hydroxylase, aldosterone synthase) Cytochrome P450, subfamily XIB, polypeptide 2 (aldosterone synthase), aldosterone synthase, steroid 11-beta/18-hydroxylase, steroid-11-beta-hydroxylase, 11-beta-hydroxylase Produces cynodontin Produces hydrocortisone cortisol Produces steroids Transforms cycloalkenones Transforms sesquiterpene lactone costunolide Hydroxylation of cortexolone (= substance S) to hydrocortisone
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format	frozen
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Type Strain	no
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Preceptrol®	no
Comments	This fungus was re-identified as Curvularia pseudobrachyspora based on the multigene DNA sequence comparison.
Medium	ATCC^® Medium 340: Rabbit food agar ATCC^® Medium 343: V8 juice agar ATCC^® Medium 336: Potato dextrose agar (PDA)
Growth Conditions	Temperature: 24°C to 26°C Atmosphere: Typical aerobic
Sequenced Data	18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence GGTCTCCGTAGGTGAACCTGCGGAGGGATCATTACACAAATTAAAATATGAAGGCTTCGGCTGGATTATTTTTATCACCCTTGTCTTTTGCGCACTTGTTGTTTCCTGGGCGGGTTCGCCCGCCACCAGGACCACACCATAAACCTTTTTTATGCAGTTGCAATCAGCGTCAGTATAACAAATGTAAATCATTTACAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATACGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAAAGGGCATGCCTGTTCGAGCGTCATTTGTACCCTCAAGCTTTGCTTGGTGTTGGGCGTCTTTTGTCTTTGGCCTCGCCCAAAGACTCGCCTTAAAACGATTGGCAGCCGGCCTACTGGTTTCGCAGCGCAGCACATTTTTGCGCTTGCAATCAGCAAAAAGGACGGCAATCCATCAAGACTACATTTTTACGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCTCTTTCAGAGTCCGAGTTGTAATTTGCAGAGGGCGCTTTGGCTTTGGCAGCGGTCCAAGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTACGTGGTCGCTAGCTATTGCCGTGTAAAGCCCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTCAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCAGCCAGACTTGCTTGCAGTTGCTCATCCGGGCTTTTGCCCGGTGCACTCTTCTGTAGGCAGGCCAGCATCAGTTTGGGCGGTGGGATAAAGGTCTCTGTCACGTACCTTCCTTCGGGTTGGCCTTATAGGGGAGACGCCATACCACCAGCCTGGACTGAGGTCCGCGCATCTGCTAGGATGCTGGCGTAATGGCTGTAAGCGGC glyceraldehyde-3-phosphate dehydrogenase (GPD) gene CACTTGCTTTGGCCCGACGCCTCGGCATCATTCATTGGTAGAGCAGTGCAGGCTAACATCTACATAGGCATACATGCTCAAGTATGACAGCACACACGGCCAGTTCAAGGGCGACATCAAGGTTGACGGCAACAACCTGACTGTCAACGGCAAGACTGTCCGCTTCCACATGGAGAAGGACCCCGCCAACATCCCATGGAGCGAGACCGGCGCCTACTACGTCGTTGAGTCCACCGGTGTCTTCACCACCACCGAGAAGGCCAAGGCTCACTTGAAGGGTGGAGCCAAGAAGGTTGTCATCTCTGCTCCTTCCGCCGATGCCCCCATGTTCGTCATGGGTGTCAACCATGAGACCTACAAGTCCGACATTGAGGTGCTCTCCAACGCCTCTTGCACAACCAACTGCTTGGCTCCTCTTGCCAAGGTCATCCACGACAAGTACACCATCATTGAGGGTCTCATGACCACCATCCACTCCTACACTGCCACCCAGAAGGTCGTTGACGGCCCCTCCGCTAAGGACTGGCGTGGTGGCCGCACCGCTGCTCAGAACATCATTCCCAGCAGCACTGGTGCCGCCAAGGCTGTCGGCAAGGTCATCCCAGAGCTCAACGGCAAGCTCACTGGTATGGCCATGCGTGTCCCTACCGCCAACGTCTCCGTTGTCGACTTGACTGTCCGCATCGAGAAGGGTGCTTCTTACGACGAGATCAAGCAGGCCGTCAAGGAGGCCTCCGAGGGCTCCCTGAGCGGTAAGTCTCTCTTTGCATCAAGATTTTATGTATACAAGCTAATACAATGCAGGTATCCTTGGCTACACCGAGGACGACATTGTCTCCACTGACCTGAACGGCGACAACCGCTCCTCCATCTTCGACGCCAAGGCCGGTATCTCCCTGAACAAGAACT
Name of Depositor	NRRL
U.S. Patent Number	4,528,271
Chain of Custody	ATCC <-- NRRL <-- Chas. Pfizer Co.
Isolation	Tarpaulin, Panama
References	Yanagihara M, et al. Tiny keratotic brown lesions on the interdigital web between the toes of a healthy man caused by Curvularia species infection and a review of cutaneous Curvularia infections. Mycoscience 51: 224-233, 2010. Sonomoto K, et al. 11beta-Hydroxylation of cortexolone (Reichstein compound S) to hydrocortisone by Curvularia lunata entrapped in photocross-linked resin gels. Appl. Environ. Microbiol. 45: 436-443, 1983. Shull GM, et al. Oxygenation of steroids. US Patent 2,658,023 dated Nov 3 1953 Santhanam HK, Shreve GS. Solvent selection and productivity in multiphase biotransformation systems. Biotechnol. Prog. 10: 187-192, 1994. PubMed: 7764676 Carnell A, Willetts A. Biotransformation of cycloalkenones by fungi Baeyer-Villiger oxidation of bicycloheptenone by dematiaceous fungi. Biotechnol Lett 12: 885-890, 1990. Chen KC, Wey HC. 11beta-Hydroxylation of coxtexolone by Curvularia lunata. Enzyme Microb. Technol. 12: 305-308, 1990. Chen KC, Wey HC. Dissolution-enzyme kinetics of 11β-hydroxylation of cortexolone by Curvularia lunata. Enzyme Microb Technol 12: 616-621, 1990. Clark AM, Hufford CD. Microbial transformations of the sesquiterpene lactone costunolide. J. Chem. Soc. Perkin Trans. 1979: 3022-3028, 1979. Canonica L, et al. The microbiological oxidation of insect moulting hormones. J. Chem. Soc. Chem. Commun. 1974: 656-657, 1974. Van Eijk GW, Roeymans HJ. Cynodontin, the tetrahydroxyanthraquinone of Curvularia and Drechslera species. Experientia 33: 1283-1284, 1977.

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