| 產品名稱 |
SU-DHL-6 |
| 商品貨號 |
B167210 |
| Organism |
Homo sapiens, human |
| Tissue |
peritoneal effusion; derived from metastatic site: peritoneal cavity |
| Cell Type |
B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast-like |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
large cell lymphoma; diffuse mixed histiocytic and lymphocytic lymphoma; follicular B cell lymphoma |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
This is a pseudodiploid cell line with a modal chromosome number of 47, a polyploidy rate of approximately 9% and one copy of the X chromosome. No Y chromosome was observed in any of the analyzed cells. A derivative t(14;18)(q32;q21) chromosome was present in most of the examined cells. Other derivative chromosomes were generally consistent with previous studies, these include: del(6)(p23), del(9)(p21.1?), add(11)(q25), i(17q) [or t(6?;17)(p10;q10)?] and del(18)(q21)?. [PubMed: 3881165] |
| Derivation |
The DHL cell lines were successfully established in continuous suspension culture from 10 patients with a histopathological diagnosis of diffuse histiocytic lymphoma (DHL). |
| Clinical Data |
43 years
Caucasian
male |
| Genes Expressed |
monoclonal cytoplasmic immunoglobulin: IgM; lambda light chain |
| Comments |
All of the DHL cell lines were negative for the presence of Epstein-Barr virus (EBV) genomes.
SU-DHL-6 possesses a t(14;18)(q32;q21) translocation and demonstrates an unexpected recombination within its heavy chain gene locus that may be the interchromosomal breakpoint.
In SU-DHL-6, the t(14;18) translocation juxtaposes a truncated bcl-2 gene with J6 in a tail-to-head configuration.
The deregulated expression of the altered bcl-2 gene may play a critical role in the disordered growth and differentiation of follicular B cell lymphoma.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by the addition of fresh medium. An inoculum of 8 X 104 to 3 x 105 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended.
Medium renewal: Every 3 to 4 days |
| Cryopreservation |
Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO
Storage Temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile |
D5S818: 12
D13S317: 12,14
D7S820: 10
D16S539: 11,12
vWA: 14,17
THO1: 6,9.3
TPOX: 11,12
CSF1PO: 10
Amelogenin: X |
| Population Doubling Time |
approximately 29 hours |
| Name of Depositor |
A Epstein |
| Year of Origin |
1977 |
| References |
Hua C, et al. Consequences of the t(14;18) chromosomal translocation in follicular lymphoma: deregulated expression of a chimeric and mutated BCL-2 gene. Oncogene Res. 2(3): 263-275, 1988. PubMed: 3285301
Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220
Kaiser-McCaw Hecht B, et al. Histiocytic lymphoma cell lines: immunologic and cytogenetic studies. Cancer Genet. Cytogenet. 14 (3-4): 205-218, 1985. PubMed: 3881165
Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039
Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794
Bakhshi A, et al. Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18. Cell. 41(3):899-906, 1985. PubMed: 3924412
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