| 產(chǎn)品名稱 |
pC194Amy |
| 商品貨號(hào) |
B223424 |
| Designations |
pC194Amy |
| Species |
Geobacillus stearothermophilus (Donk) Nazina et al. |
| Depositors |
CPC International, Inc. |
| Applications |
The genotype for the host strain is phe tyr trp sacA. |
| Vector |
Construct size (kb): 8.300000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pC194
Intact vector size: 2.900
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: HaeIII HindIII BglI
Polylinker sites:
Construction: natural isolate
Host range: Bacillus sp.; Staphylococcus aureus
Features (with orientation and position when available):
marker(s): cmlR
replicon: pC194
Cross references: DNA Seq. Acc.: L08860 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pC194
Intact vector size: 2.900
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: HaeIII HindIII BglI
Polylinker sites:
Construction: natural isolate
Host range: Bacillus sp.; Staphylococcus aureus
Features (with orientation and position when available):
marker(s): cmlR
replicon: pC194
Cross references: DNA Seq. Acc.: L08860 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus stearothermophilus
Gene name: alpha-amylase
Contains complete coding sequence?: U
Strain: N21
Type of DNA: genomic
Insert end: HindIII
Insert end: HindIII
Insert size (kb): 3.0
Cross references: DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus stearothermophilus
Gene name: alpha-amylase
Contains complete coding sequence?: U
Strain: N21
Type of DNA: genomic
Insert end: HindIII
Insert end: HindIII
Insert size (kb): 3.0
Cross references: Insert lengths(kb): 3.0 Gene product: amylase, alpha 1A; salivary(Amylase, salivary, alpha-1A) |
| Insert Size (kb) |
3.0 |
| Media |
ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
The genotype for the host strain is phe tyr trp sacA. Plasmid encodes a thermostable alpha-amylase. The probe was constructed from pC194 and Bacillus stearothermophilus plasmid DNA. |
| References |
Mielenz JR, Mickel S. Process for cloning the gene coding for a thermostable alpha-amylase into Escherichia coli and Bacillus subtilis. US Patent 4,493,893 dated Jan 15 1985
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
