| Insert |
DNA: genomic Insert lengths(kb): 17.0 Tissue: liver Gene product: DNA Segment, single copy [D21S112] Alleles: A1, A2, A3, A4, A6, A7, A8, A9, B1, B2, B3, B5, B6, B8, A11, A12, A13, A14, A16, B10, B11, B12, B7, A5, B9, A10, A15, B4 |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII-->23.0, 18.0, 5.8; KpnI--19.0 (doublet), 6.4, 1.5; PvuI--16.0, 14.0, 5.2, 2.5; SacI--23.0, 8.0, 5.0, 3.6 (doublet), 3.0, 1.8; EcoRI--20.0, 12.0, 5.2 (doublet), 3.8, 2.1, 0.6, 0.4. The RsaI polymorphisms have a combined PIC value of greater than 0.93 and a combined heterozygosity of greater than 95%. |
| References |
Schumm JW, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am. J. Hum. Genet. 42: 143-159, 1988. PubMed: 2892396
Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638
Kazazian HH Jr., et al. Characterization of a spontaneous mutation to a B-thalassemia allele. Am. J. Hum. Genet. 38: 860-867, 1986. PubMed: 3014870
Knowlton RG, et al. Use of highly polymorphic DNA probes for genotypic analysis following bone marrow transplantation. Blood 68: 378-385, 1986. PubMed: 3524708
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