| Comments |
EcoRI digest contains fragments of about 20, 11, 6.8, 3.2, 2.6, 1.23, 1.15 and 1.07 kb. Restriction digests of the clone give the following sizes (kb): BamHI--29, 6.4, 5.6, 4.0, 1.6, 0.54; EcoRI--20, 11, 6.8, 3.2, 2.7, 1.3, 1.25, 1.1; HindIII--20, 5.8, 5.8, 4.25, 3.25, 2.7, 2.3, 1.9, 1.15; SalI--uncut; XbaI--20, 15, 12. This genomic clone contains the entire coding region of proenkephalin. To block repeated sequences in the probe, prehybridize with sonicated total human placental DNA instead of salmon sperm DNA. Final stringency wash with 0.1X SSC/0.1% SDS at 65-68C. Enzyme(s) not detecting polymorphism: 29 enzymes including TaqI, MspI, RsaI, BamHI, PvuII, PstI. |
| References |
Comb M, et al. Primary structure of the human proenkephalin gene. DNA 2: 213-229, 1983. PubMed: 6315326
Litt M, et al. Chromosomal localization of the human proenkephalin and prodynorphin genes. Am. J. Hum. Genet. 42: 327-334, 1988. PubMed: 2893547
Litt M, Buder A. A frequent RFLP identified by a human proenkephalin genomic clone (HGM9 symbol PENK). Nucleic Acids Res. 17: 465, 1989. PubMed: 2783483
James Douglass, personal communication
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