| Vector |
Construct size (kb): 0.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene symbol: PHO3,5
Contains complete coding sequence?: U
Chromosome: II; Localization: II R, 57 cM from CEN
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb):
Cross references: Gene product: [PHO3,5] |
| Comments |
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 0.695, 0.870, 2.008, 8.185, 1.047, 1.675, 1.113, 2.319, 1.636, 1.238, 0.566, 0.560, 0.428, 1.118, 4.115, 0.848, 0.639, 4.963. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70135, 70362, 70404, 70687, 70768, 70930. NOTE: Because of the large number of simultaneous deposits from Dr. Olson, the ATCC has not verified that the characteristics of the material supplied here are as published. The ATCC encourages users of this material to communicate their results to us. |
