Restriction digests of the clone give the following sizes (kb): EcoRI/PstI--4.6, 2.8; HindIII--7.4; SalI--7.4. The insert contains the following restriction sites (approximate from the 5' end): BstXI--3.95; NcoI--2.52; SalI--2.48; SphI--2.42, 2.76, 3.65; XhoI--2.79. The insert contains the following components (listed 5'->3'): a PstI/SalI fragment from the MCS of lambda EMBL3, 2.4 kb upstream of the coding sequence for DHFR-TS, the 1.56 kb coding region, and 0.44 kb downstream of DHFR-TS. All but 100 bp of the coding sequence is in a 1.9 kb SalI/EcoRI fragment. |
