| Insert |
DNA: genomic Insert lengths(kb): 2.700000047683716 Gene product: alpha-1-antitrypsin (protease inhibitor) [PI] Alleles: G3, A1, A2, B1, B2, D2, G1, G2, J2, N1, N2, C1, C2, D1, J1 Target Gene: alpha-1-antitrypsin (protease inhibitor) |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--5.1, 2.75 (doublet), 0.70; EcoRV--11.5; HindIII--5.8, 4.5, 1.05. The insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--2.26. A minigene constructed from genomic sequence beginning 50 bp upstream of the start ATG (exon 2) and extending into exon 3, ligated to a cDNA fragment encoding exons 3-5. |
