| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--11.5, 10.5, 7.4, 6.6, 6.2, 2.7, 1.9, 1.0, 0.64; EcoRI--13.0, 11.5, 9.8, 9.3, 3.9, 1.9; BamHI--13.0, 12.0, 7.0, 5.9, 3.7; PstI--10.0, 8.6, 3.35, 2.25, 1.7, 1.4, 1.3, 1.2; HindIII--15.0, 13.0, 10.0, 4.7, 3.6, 3.0; consistent with deposited DNA. Codominant segregation was demonstrated in 60 families. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. |
| References |
Harris PC, et al. A long-range restriction map between the alpha-globin complex and a marker closely linked to the polycystic kidney disease 1 (PKD1) locus. Genomics 7: 195-206, 1990. PubMed: 2347584
Julier C, et al. A primary map of 24 loci on human chromosome 16. Genomics 6: 419-427, 1990. PubMed: 2328986
Raymond L White, personal communication
|
