Restriction digests of the clone give the following sizes (kb): HincII--4.2; HindIII--3.1, 1.15; PvuII--2.4, 0.95 (doublet); XhoI--2.7, 1.4; SmaI--4.2; EcoRI/PstI--2.6, 1.6. The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.44; PvuII--0.71; ClaI--0.89, 1.36; XhoI--0.95; SmaI--1.39. Contains nucleotides 1893-3402, which includes the 3' end of the ORF and the 3' untranslated region. |
