Some restriction sites not found in the insert: BamHI BglI BglII EcoRI HaeII HincII KpnI NcoI NotI PvuI PvuII SacI XbaI XhoI. Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.7, 0.9; BglI--2.4, 1.2; HindIII--3.0, 0.5; PstI--2.8, 0.75; SmaI--3.5; PvuI/HindIII--1.6, 0.96, 0.66, 0.43; BglI/HindIII--1.4, 1.1, 0.66, 0.43. The insert contains the following restriction sites (bp from the 5' end): AvaI--86, 96; HindIII--477; PstI--166; SmaI--96. The insert is a pcr product amplified from human placental DNA. The insert is in the opposite orientation as lacZ'. |
